Device and method for determining the stability of substances and formulations

ABSTRACT

The invention relates to a novel device for determining the physical and chemical stability of substances and formulations by means of samples. Said device is provided with a vibration spectrometer, a container ( 1 ) for storing the samples in controlled conditions, a robot ( 10 ) for transporting the samples between the vibration spectrometer ( 12 ) and the container, and a computer ( 13 ) for controlling the processes and for automatically storing and evaluating the spectra of the samples received by the vibration spectrometer.

[0001] The present invention relates to a device according to the preamble of claim 1 and a method according to the preamble of claim 6.

[0002] The present invention relates to a device and a method for determining the physical and chemical stability of substances and formulations on the basis of spectral data which, with the aid of suitable vibration spectrometers (middle-infrared, near-infrared, Raman spectrometers), may be recorded non-destructively completely automatically in short time intervals (minutes, hours, days) from substances and formulations stored under controlled conditions (temperature and/or ambient humidity and/or light).

[0003] Typically, physical and chemical stabilities of substances and formulations are determined in such a way that the substances and formulations are stored in controlled storage conditions for a long period of time. At the beginning of the storage and at long time intervals (several weeks, months, years), samples are taken from the stored substances and formulations, which are subsequently assayed in a laboratory using physical and chemical analyses (chromatographic, electrophoretic, wet-chemical, and physical methods). These methods are distinguished by various disadvantages:

[0004] The performance of the classical physical and chemical analyses typically requires a large outlay in time since, for example, complex sample preparations are necessary for chromatographic and electrophoretic analyses.

[0005] To perform chromatographic analyses, it is typically necessary for reference material having a known content of the substances to be assayed to be present.

[0006] The material taken from the stored substances and formulations for the analyses may no longer be used for further storage, since the samples are destroyed in most cases in the classical physical and chemical analyses.

[0007] Because of the great complexity, the analyses may only be performed at long time intervals. In comparison to the present invention, it takes significantly longer to obtain conclusions about the stability of the stored substances and formulations from the experimental data.

[0008] For example, a device for determining types of adsorbates, in which an infrared spectrometer disperses a beam of radiation from a catalyst which a gas is subjected to for analysis in order to adsorb adsorbates thereon, is known from DE-A-197 44061. The spectrometer outputs spectral data, corresponding to a wave number of the beam of the radiation, to a computer which has reference data stored. The computer standardizes the spectral data and the reference data and then calculates a product of the standardized spectral data and the reference data. A function of the product in relation to the wave number is then differentiated in order to obtain a differential function. Then, a specific wave number is determined for which the differential function is equal to zero, so that the shared peak of the spectral data and the reference data at the specified wave number is determined precisely.

[0009] The present invention is thus based on the object of specifying a device and a method, using which confirmed information about the physical and chemical stability of substances and formulations is provided in a very rapid and non-destructive way.

[0010] This object is achieved by a device having the features of claim 1 and by a method having the features of claim 6.

[0011] Further advantages of the present invention result from the dependent claims and the following description, in which the present invention is described in greater detail on the basis of an exemplary embodiment illustrated in the schematic drawing.

[0012]FIG. 1 shows a schematic illustration of a measurement device,

[0013]FIG. 2 shows a flowchart of the analysis,

[0014]FIG. 3 shows a scatter diagram of the main components,

[0015]FIG. 4 shows a diagram of the main components weighted with the coefficients of the regression calculation,

[0016]FIG. 5 shows a diagram to illustrate the alteration from the scores, and

[0017]FIG. 6 shows a sample container having an evaporation device, and

[0018]FIG. 7 shows a sample vessel having multiple depressions and an evaporation device for multiple sample containers.

[0019]FIG. 1 shows, purely schematically, a transport robot 10, known per se in chemical laboratories, which may be moved back and forth between a storage container 11 and a spectrometer 12 by a computer 13. The samples are transported by the transport robot 10 in a sample vessel (not shown) from the storage container 11 to the spectrometer 12, where the spectra are recorded. Subsequently, the robot 10 transports the samples back to the storage container 11 again. The movement sequences are now programmed using the computer 13 in such a way that the measurements are performed at the same measurement conditions (temperature, incident light, relative humidity).

[0020] To perform physical and chemical stability studies, in the method described here, vibration spectra from a sample of the substance or formulation stored in defined storage conditions are recorded automatically under identical measurement conditions using the spectrometer 12 in regular time intervals (minutes, hours, days). Middle-infrared, near-infrared, and Raman spectroscopy are vibration spectroscopy methods suitable for these studies. The recorded spectra of each sample are stored in the computer 13 as raw data, each spectrum being provided with the sample number, the storage conditions, and the time of the measurement using an index. The analysis of the raw data is performed through statistical data analysis. It begins for each sample automatically as soon as the number of spectra stored on the computer 13 is sufficient for a reasonable analysis and is recalculated as soon as new spectra of the sample are recorded. The current results of the analysis may be accessed at any time.

[0021] The execution of the automatic data analysis occurs according to the method indicated in the scheme in FIG. 2. At the start 20, the measurement is initiated by the computer 13. The first step 21 includes producing a data set which includes all spectra S of a sample at specific storage conditions, according to which a vector S is produced from the spectra entered in the computer 13. In step 22, a data transformation S→S′ is then performed, by subtracting a baseline from each spectrum in the vector S, for example. The analysis begins with the spectra of the data transformation of all spectra in the data set which contain a baseline correction or a vector standardization, for example. The second analysis step 23 includes the analysis of the main components or similar transformations of all transformed spectra S′ of a sample. The scatter diagram 24 of the main components may be graphed from this. With the aid of at least one of the calculated main components and the time of the measurement of each spectrum, the systematic temporal change of the spectra is subsequently determined with the aid of a regression calculation 25 of the main components in accordance with the formula t=B*HK+e, t being the vector from the storage time, B being the vector having the regression coefficients, HK being the matrix having the main components, and e being the vector having the experimental errors. The spectral representation 26 of the sums of the main components HK weighted with the regression coefficients then results therefrom. The results obtained from the regression calculation allow qualitative and quantitative statements about the physical and chemical stability of the sample. The projections or scores may be calculated (step 27) from the main component regressions 25, from which the scores may be graphed as a function of the storage time (step 28). After the calculation of the scores, the method is ended at step 29 and the measurements are continued on the next sample.

[0022] In FIG. 3, the various main components 1.HK, 2.HK, 3.HK, and 4.HK are represented in the form of a scatter diagram in each case. These allow conclusions about the number of independent processes occurring during the storage of the sample. In this case, the individual spectra are represented in the diagrams as points which are connected in temporal sequence using a line. Particulars may be inferred from the book “Statische Datenanalyse -Eine Einfuhrung fur Naturwissenschaftler [Statistical Data Analysis—an Introduction for Physical Scientists]” by Werner A. Stahel, Vieweg 1995, about the main components on pp. 307 and 308, about scatter diagrams in chapter 3.1, particularly pp. 34 to 36, and about the matrix of scatter diagrams in chapter 3.6, pp. 48 to 51.

[0023] A diagram of the sum of the main components (or process factors) weighted with the coefficients of the regression calculation is shown in FIG. 4. The wavelength λ is plotted on the x-axis, the y-axis is dimensionless and shows the intensity changes. At a wavelength of approximately 1450 nm, there is a significant decrease of the intensity, from which a decrease of the concentration of the vibration oscillations of the associated molecule, and therefore reduced storability, may be concluded. The main components weighted with the coefficients of the regression calculation thus show the base vector for the temporal drift of the spectra and have the same physical units as these. The process factor allows conclusions to identify the materials participating in the physical and chemical alteration processes.

[0024] The associated scores are obtained through mathematical projection of the transformed spectra onto the calculated process factor. The scores are shown in the temporal sequence in FIG. 5. Together with the time t of the measurement of the spectra, they allow conclusions about the kinetics of the physical or chemical alteration processes. In connection with the Lambert-Beer law, the extent of the alteration (e.g., the chemical degradation) may be established quantitatively from the scores, if the process may be uniquely assigned to a substance present in the sample from the associated loadings.

[0025] A sample vessel 30 having a cover 32 tightly sealed by seals 31 is shown in FIG. 6. An overflow Channel 33 is provided in the cover 32, which is connected on two diametrically opposite sides to hoses (not shown here) via connections 34. Using this sample vessel, the samples may be vaporized in a controlled way. For this purpose, the sample vessel 30 is sealed using the cover 32 after pouring in the sample liquid and the hoses are connected. The overflow channel 33 then has a protective gas flow over it in a regulated and/or constant gas flow during the storage and/or the evaporation of the solvent. The measurements of the vibration spectra then occur in the sample vessel 30. In addition, the sample vessel 30 may also be sealed hermetically using a suitable seal 36 (see FIG. 6a), which includes a mechanical closure 37 (e.g., stopper or twist closure) and an artificial resin layer 38, poured over it and cured, which hermetically seals the closure 37.

[0026] A further embodiment of a sample vessel 40 is illustrated in FIG. 7, which includes a microtest plate 41 having multiple depressions 42 and an evaporation device 43 having multiple cover-like caps 44 and an overflow channel 46 having connections 47. Such a microtest plate 41 is preferably made of quartz glass and is described, for example, in the catalog 66/97-1 of HELLMA GmbH & Co. D-79371 Mullheim, on page 155. The microtest plate 41 is now sealed line by line with the evaporation device 43, designed as a cover. The overflow channel 46 subsequently has a protective gas flow over it at a regulated and/or constant gas flow during the storage and/or the evaporation of the solvent, which flows via hoses (not shown here) connected to the connections 47. The measurement of the vibration spectra (NIR, Raman) now occurs in the particular depression from below and/or, after the complete evaporation of the solvent and the removal of the evaporation device 43, from top to bottom in the transmission mode, or using an infrared and/or Raman microscope. 

We claim:
 1. A device for determining the physical and chemical stability of substances and formulations using samples, characterized in that a vibration spectrometer, a container (11) for storing the samples under controlled conditions, a robot (10) for transporting the samples between the vibration spectrometer (12) and the container, and a computer (13) for controlling the sequences and for automatic storage and analysis of the spectra of the samples recorded using the vibration spectrometer are provided.
 2. The device according to claim 1, characterized in that the vibration spectrometer (12) is a middle-infrared, near-infrared, or Raman spectrometer.
 3. The device according to claim 1 or 2, characterized in that the container (11) has sensors for measuring and maintaining the control conditions.
 4. The device according to claim 3, characterized in that a light sensor and/or a temperature sensor and/or a humidity sensor and/or a sensor for measuring the gas atmosphere in the container are provided.
 5. The device according to claim 1 or 2, characterized in that the container (30; 40) is provided with an evaporation device (32; 43), having an overflow channel (33; 46) , in order to be able to vaporize the samples under controlled conditions.
 6. The device according to claim 1 or 2, characterized in that the container (30) is hermetically sealed by a seal (36).
 7. The device according to claim 6, characterized in that the seal (36) has a mechanical closure (37) and an artificial which seals this closure.
 8. A method for determining the physical and chemical storage stability of substances and formulations, in which vibration spectra of samples of the substances and formulations are recorded at regular time intervals, characterized in that each individual vibration spectrum of a sample is indexed using the sample number, the storage conditions, and the measurement time, the recorded and indexed vibration spectra are stored in a computer as raw data, and, as soon as the number of stored spectra has exceeded a predetermined threshold value, the raw data of each individual sample is analyzed using statistical data analysis.
 9. The method according to claim 8, characterized in that in each case a data set is produced from all spectra of an individual sample and the spectra are analyzed through a data transformation in the data set.
 10. The method according to claim 9, characterized in that the data transformation contains a baseline correction or a vector standardization.
 11. The method according to one of claims 9 or 10, characterized in that all transformed spectra of an individual sample are analyzed in a main component analysis or a similar transformation and the systematic temporal alteration of the spectra is established using regression calculation of the main components. 